摘要
经60 Coγ射线辐照处理获得的诱变菌株芽孢杆菌BS9418F ,其产酶活力比出发菌株提高 30 %以上。该菌株以大麦粉 7%、玉米粉 3%、豆粕 3%及适量无机盐为培养基最佳配比 ,其最适培养条件为 :培养基初始 pH 7.0 ,摇瓶装量 5 0mL/ 30 0mL三角瓶 ,种龄 16～ 2 0h ,接种量 2 %～ 3% ,培养温度 36～ 37℃ ,发酵周期 40h。在优化条件下 ,摇瓶发酵产 β 葡聚糖酶活力高达 5 5 0 0u/mL以上 ,比出发菌株初始发酵水平提高了
After exposed in γ-ray of+{60}Co, a mutant strain of Bacillus subtilis 9418 was obtained.The enzyme activity of the strain increased 30% more than original one. The optimum proportions of medium, fermentation conditions were studied. Under optimized conditions, the activity of the enzyme in shaker flask reached up to 5 500 μ/mL raising 4 folds of the original strain.
出处
《微生物学杂志》
CAS
CSCD
2002年第1期29-31,共3页
Journal of Microbiology
基金
浙江省重大攻关项目计划专题资助 ( 96 0 2 )
