摘要
紫球藻经Nd :YAG激光 (波长 10 6 0nm)照射 ,以不同时间设置处理剂量 ,比较紫球藻细胞活力、生长速率、生物量、胞外多糖产量等方面的变化。高剂量处理组 (Y - 2 0、Y - 30和Y - 40 )致死率为 17～ 44 % ,继代培养后生长迅速 ,K值分别比对照组提高 18%、2 3.4%和 15 .3%。各处理组培养 10天后收获的生物量与对照组无显著差别 ,但胞外多糖产量均有较大幅度提高 ,增幅达 5 0～ 15 0 %。此外 ,YAG激光照射对藻细胞叶绿素含量也有影响。YAG激光有望成为紫球藻优良藻株选育的较佳诱变剂。
Porphyridium cruentum was irradiated with Nd∶YAGl laser(wavelengh 1060?nm) by different time as treatment dose. After irradiated,the activity of cells,growth rate,biomass and the extracellular polysaccharides yield of Porphyridium cruentum were investigated to analyse Laser irradiated phenomenon. The experimental results showed that the lethally rate of higher irradiated does(20sec;30sec and 40sec) preponderated over the low dose,as to be from 17%to 44%. The higher dose irradiated cells growth raptly in continue cultivation,growth rate K value increased 18%?23.4% and 15.3% respectively to that of CK. Physiological and biochemical characteristic of cells after Laser treated were determined. Anong those,extracelluar polysaccharides yield improved most,by 50%～150% than that of CK. Pigment content was varied at different irradiated dose. But biomass of treatment cells incubatd 10 days have no prominent distinction from the control. All results revealed that Nd∶YAG Laser can be a hopeful mutagen in Porphyridium cruentum breeding.
出处
《激光生物学报》
CAS
CSCD
2002年第1期6-9,共4页
Acta Laser Biology Sinica
基金
福建省自然基金 (No :B0 0 2 0 0 0 1)
福建省计委基金资助项目
关键词
紫球藻
倍频ND:YAG激光
生长
胞外多糖
Porphyridium cruentum
Nd∶YAG laser
Growth
Extracellular polysaccharide
