摘要
以快速定量滤纸为基质 ,用KI NaAc为重原子微扰剂 ,建立了测定痕量可可碱、咖啡因、茶碱的滤纸基质室温磷光 (PS RTP)分析法。该法取样量少 ,线性范围宽 ,可可碱、咖啡因、茶碱的线性范围分别为 14 4～5 76 ;5 .4 4～ 6 99;7.2 1～ 36 0ng/斑点 ;检出限分别为 1 14、0 78、1 80ng/斑点。该方法用于巧克力中可可碱、茶叶中咖啡因、氨茶碱药片中茶碱的测定 ,操作简便快速 ,选择性好。标准回收率在 92 8%～ 10 6 %之间 ;相对标准偏差RSD <5 %
Theobromine, caffeine and theophylline adsorbed on filter paper can emit strong room temperature phosphorescence (RTP) signal in the presence of heavy atom perturber, the RTP signal was used for the determination of these compounds. The method is based on using quantitative fast speed paper as a substrate and KI NaAc as heavy atom perturber. Various factors affecting the RTP signal were discussed. The method is a combination of trace analysis with microtechnique using 4 μL sample. The linear dynamic ranges for theobromine, caffeine and theophylline are from 14.4 to 576, 5.44 to 699 and 7.21 to 360 ng per spot, respectively. The recovery of standard added to commercial products of chocolate, tea and aminophylline is in the range 92.8%～106%. Compared with the results obtained by standard methods, the experimental results are satisfactory. The method is simple, rapid and sensitive and can be applied to the analysis of commercial products without interference.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2002年第3期301-303,共3页
Chinese Journal of Analytical Chemistry
基金
国家自然科学基金 (No .2 9875 0 16)
山西省青年科学基金 (No .2 0 0 110 10 )资助项目
