摘要
目的：研究制备抗人喉癌／抗蓖麻毒素Ａ双功能抗体用以导向喉癌的治疗。方法：在制备抗人喉癌单克隆抗体杂交瘤细胞基础上，通过二次杂交瘤技术制备能够分泌抗人喉癌和抗蓖麻毒素Ａ的杂交瘤细胞株。结果：该双功能抗体对Ｈｅｐ－２细胞株杀伤力比化学偶联法的双功能抗体强１５倍。结论：采用二次杂交瘤技术制备的双功能抗体比化学偶联法的细胞毒性作用显著增高，优于化学偶联法。
Objective: To study the preparation of bifunctional antibody against human laryngeal cancer/anti-castor toxin-A and its cytotoxity activity for tumor cell-Methods:On the basis of preparation in monoclonal antibody against human laryngeal carcinoma,the hybridoma cell of anti-human laryngeal carcinoma/anti-castor toxin-A was prepared by the technique of secondary hybridoma.The secret bifunction antibody of the secondary hybridoma cell line was demonstrated by ELISA method.Result:The killing effect of the bifunctional antibody to Hep-2 cell line was 15 times stronger than that in the chemical coupling bifunctional antibody.Conclusion:Contrasting with chemical coupling bi functional antibody,the cytotoxity effect of bifunctional antibody which prepared by the technique of secondary hybridoma was obviously increased.The techniqueof secondary hybridoma was superior to one of chemical coupling bifunctional antibody. [Chinese Arch Otolaryngol Head Neck Surg,2002;9(1):36～38 From the Department of Otolaryngology-Head and Neck Surgery,Second Hospital of Jilin University,Changchun 130041(Dr.Sun Liqun)]
出处
《耳鼻咽喉（头颈外科）》
2002年第1期36-38,共3页
Chinese Arch Otolaryngology-Head Neck Surg
基金
国家自然科学基金资助项目
课题号3940747
