摘要
目的 制备抗人甘露聚糖结合凝集素单克隆抗体及建立应用方法。方法 用亲和层析法自人血清中提取甘露聚糖结合凝集素 (MBL) ,免疫Balb/c小鼠 ,通过细胞融合的方法 ,得到 3株稳定分泌抗人MBL单克隆抗体 (mAb)的杂交瘤细胞株。建立检测MBL的ELISA。结果 本组mAb识别相对分子质量 (Mr)为 310 0 0的MBL分子 ,与MBL作用后可明显抑制MBL 甘露聚糖复合物的抗补体效应。用ELISA检测 186例健康人血清中MBL水平为 2 6 9± 1 5 8mg/L。结论 成功地获得了抗MBL的mAb并用于建立ELISA。
Objective To prepare monoclonal antibodies (mAbs) to mannan binding lectin (MBL) and develop a method for detection by using it Methods Mannan binding lectin in human serum was isolated by affinity chromatography and used as antigen to immunize Balb/c mice Three hybridome cell lines that could steadily secret antiMBL mAb were established The mAbs were used for ELISA Results The mAbs specifically recognized MBL molecule with Mr 31 000 and markedly inhibited the effect of anti complement of MBL mannan complex The MBL levels (2 69±1 58 mg/L) in 186 sera from normal subjects were detected by ELISA Conclusion The monoclonal antibodies to mannan binding lectin were successfully prepared and used for ELISA
出处
《临床检验杂志》
CAS
CSCD
北大核心
2001年第6期325-327,共3页
Chinese Journal of Clinical Laboratory Science
