摘要
目的 用PCR和酶联试验鉴别布氏菌与耶尔森氏菌感染。方法 以不同种布氏菌及不同型耶尔森氏菌分别感染动物 ,不同时间采样进行PCR和DAgS -ELISA及其他血清学方法进行检查。结果 两菌感染血清的SAT都出现了不同程度的交叉 ,RBPT及DAgS -ELISA几乎无交叉反应。布氏菌与耶尔森氏菌O∶9型的B .abortus 36KD蛋白基因引物的PCR有明显交叉反应。结论 用RBPT或DAgS -ELISA对两菌感染的鉴别是有益的。用B .abortus 36KD蛋白基因引物的PCR鉴别两菌感染是困难的 ,而且有交叉扩增。
Objective A study on the infectious identification of Brucella and Y.enterocolitia with PCR and serological tests.Methods The animal Specimentss infected by different Species and types of Brucella or Y.enterocolitia were examined with PCR?DAgS-ELISA and other serological tests in several times after infection.Results The SAT in sera of animals infested by both bacteria present the cross reactions with different degree,but RBPT or DAgS-ELISA occur almost no cross reaction.PCR of primers of 36 KD protein gene for B.abortus show Sigenificantly the cross reaction for infections samples of both bacteria.Conclusion RBPT or DAgS-ELISA is a favourable for identification of Brucella and Y.enterocolitia O∶9,but PCR of primers of 36 KD protein gene in B.abortus is a difficult for identification diagnosis.The PCR present the cross reactions of amplification for samples infected by Brucella or Yersinia.
出处
《中国地方病防治》
2002年第1期4-6,共3页
Chinese Journal of Control of Endemic Diseases
