摘要
本文为以Fmoc(Nα-芴甲氧羰基 )固相合成法制备的胸腺素α1粗品提出了一条实验室规模的液相色谱法纯化路线 ,共分两步 :第一步采用DEAESepharoseFastFlow填料进行离子交换层析操作 ,分离除去粗品中的大部分杂质 ;第二步用制备型反相高效液相色谱 (用C1 8色谱柱 )进行最后的纯化。终产物纯度可达 95% ,生物活性符合实验指标。
In this paper,a purification route using the liquid chromatogram on a loboratory scale was established on thymosin α1 synthesized by the solid-phase synthetic method using Fmoc group fo protecting α-amino acid.Two steps were included in the purification route.First was the Ion-exchange chromatography (IEC) using DEAE Sepharose Fast Flow as the matrix.After this step,majority of impure peptides was removed.Second was the preparation RP-HPLC on C18 column.Then the final product's purity attained to 95% and the values of the biological activity all meet the requirements.Those operation conditions during the separation and purification process were also studied and optimized in this thesis.
出处
《化学工业与工程》
CAS
2001年第6期331-335,353,共6页
Chemical Industry and Engineering
基金
天津市自然科学基金资助项目 (99380 4 311)资助
关键词
胸腺素Α1
固相合成多肽
离子交换层析
反相高效液相层析
分离纯化
thymosin α1
solid-phase peptide synthesis
ion-exchange chromatography (IEC)
reversed-phase high performance chromatography (RP-HPLC)
