摘要
目的 :检验对培养的牛RPE细胞的氧化损伤是否依赖于氧自由基的浓度。方法 :建立牛RPE细胞培养体系 ,利用次黄嘌呤 /黄嘌呤氧化酶 (Hypoxanthine/Xanthineoxidase ,HX/XO)系统产生牛RPE细胞氧化损伤模型。利用MTT法观察氧自由基对培养的RPE细胞增殖的影响。结果 :单独使用HX和XO对RPE细胞生长均无明显影响。当HX和XO联合使用后 ,产生大量的氧自由基 ,牛RPE细胞受到明显的氧化损伤 ,细胞生长被抑制 ,甚至导致细胞死亡 ,并且这种抑制作用对XO有剂量依赖性 ,浓度越高 ,OD值越低。结论 :HX/XO对体外培养的牛RPE细胞的生长有抑制作用。
Objective:To investigate the damage effect of oxygen free radicals on the cultured bovine retinal pigment epithelial cells.Methods:The experimental model was based on bovine RPE cells which were maintained in monolayer culture.Hypoxanthine/Xanthine oxidase (HX/XO) served as the radical generating system.The effect of HX/XO on the growth of bovine pigment epithelial cells was determined by MTT method.Results:The growth of bovine RPE cells was not influenced by HX or XO,but when HX was combined with XO,the growth of PRE cells was inhabited,meanwhile dose related reduction of cell growth was found with HX/XO treatment.Conclusion:HX/XO can inhibit the growth of bovine RPE cells in vitro.
出处
《眼科》
CAS
2002年第1期33-34,共2页
Ophthalmology in China
基金
北京市自然科学基金资助项目 ( 7962 0 0 7)
关键词
眼色素上皮
自由基
增殖
PRE细胞
pigment epithelium of eye
free radicals
proliferation
