摘要
肿瘤坏死因子与γ干扰素具有非常相似的生物学活性,并在一些主要的生物活性方面表现有较强的协同作用。本文采用寡核苷酸指导下的定位缺失-插入体外基因突变技术,删除了干扰素与肿瘤坏死因子串联基因之间的非编码区,去徐了γ干扰素的翻译终止信号,插入了一个人工设计的连接肽,使γ干扰素与肿瘤坏死因子基因在不改变读码框架的条件下融合起来,并在大肠杆菌表达系统中表述了兼有γ干扰素和肿瘤坏死因子功能的融合蛋白。
Both tumor necrosis factor and interferon gamma Share and may work synergitically in, the activities of antivirus, antitumor and immunomodulation we fused the interferon-gamma gene and tumor necrosis factor gene in frame, with a deletion of 480 bp including translational terminator and an insertion of an artificial linking sequence in between.The fused gene was expressed in E.coli anda bif unctional hybrid protein was obtained.
关键词
双功能蛋白
肿瘤坏死因子
Γ干扰素
Bifunctional fusion protein
Site-specific deletion insertion mutagenesis
