摘要
目的 观察咪达唑仑对血管张力的直接影响 ,并探讨其作用机制。方法 采用内皮完整和去内皮的大鼠离体主动脉环 ,首先观察咪达唑仑在 3× 10 -6,10× 10 -6,30× 10 -6,10 0× 10 -6mol/L浓度时 ,对去氧肾上腺素 (10 -6mol/L)引起收缩的血管环等长张力的影响。然后观察咪达唑仑对经亚甲蓝 (10 -5mol/L)孵浴处理的内皮完整血管环和分别经过维拉帕米 (5× 10 -6mol/L)和四乙胺 (5×10 -3 mol/L)孵浴处理的去内皮血管环张力的影响。结果 以上浓度的咪达唑仑对去内皮和内皮完整血管环均有舒张作用。去内皮血管环的舒张反应低于内皮完整血管环 (P <0 0 5 )。用亚甲蓝处理的内皮完整环的舒张反应与未经处理时比较无显著性差异 (P >0 0 5 )。四乙胺可增强咪达唑仑对去内皮血管环的舒张作用 (P <0 0 5 )。维拉帕米不影响咪达唑仑对去内皮血管环的舒张 (P >0 0 5 )。结论 咪达唑仑对血管具有内皮依赖性和非内皮依赖性的舒张作用 ,其内皮依赖性的舒张反应与NO sGC
Objective To investigate the direct effects of midazolam on vascular tension and the possible mechanism Methods Male Wistar rats weighing 200 250g were decapitated and the thoraco abdominal sect of aorta was removed and placed in modified Kreb's solution balanced with 95% O 2 and 5% CO 2 After removal of surrounding connective tissue and fat, the aorta was cut into rings 3 4mm in length The endothelium of some of the aorta rings were denuded Aorta rings were suspended in a 37℃ bath of Kreb's solution which was continuously balanced with 95% O 2 and 5% CO 2 One end of the ring was fixed and the other end was connected to a tension monitor through a tension transducer The intactness of endothelium was tested and confirmed by addition of phenylephrine(PE) first and then acetylcholine Aorta rings of each animal were divided into four groups: endothelium intact control group(Ⅰ); endothelium intact experiment group(Ⅱ);endothelium denuded control group(Ⅲ);and endothelium denuded experiment group(Ⅳ) The experiment was carried out in two parts: Part Ⅰ: the effect of midazolam (3×10 -6 ,10×10 -6 ,30×10 -6 ,100×10 -6 mol/L) on endothelium intact and denuded aorta rings which were precontracted with PE(10 -6 mol/L); part Ⅱ: before different concentrations of midazolam was added, the endothelium intact rings were pretreated with methylene blue(10 -5 mol/L) and the endothelium denuded rings were pretreated with verapamil(5×10 -6 mol/L) or teraethylammonium(TEA,5×10 -3 mol/L).Results The four concentrations of midazolam examined produced relaxation in both endothelium intact and denuded rings Relaxation of endothelium denuded rings was less than that of endothelium intact rings (P<0 05) There was no significant difference in the relaxation produced by midazolam between methylene blue pretreated and untreated endothelium intact rings(P>0 05) When pretreated with TEA, midazolam produced greater relaxation in endothelium denuded rings(P<0
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2001年第7期414-416,共3页
Chinese Journal of Anesthesiology
