摘要
目的观察正常人及圆锥角膜患者角膜组织中Ⅳ、Ⅴ型胶原的表达，探讨基底膜损伤在圆锥角膜病变中的作用。方法采用免疫组织化学ＳＡＢＣ法检测角膜组织中Ⅳ、Ⅴ型胶原的表达。结果正常角膜组织中Ⅳ型胶原表达在Ｂｏｗｍａｎ’ｓ层及Ｄｅｓｃｅｍｅｔ’ｓ层，圆锥角膜患者Ⅳ型胶原表达也在Ｂｏｗｍａｎ’ｓ层及Ｄｅｓｃｅｍｅｔ’ｓ层，但其阳性表达因角膜病变的程度不同而呈不同程度的减弱。Ｂｏｗｍａｎ’ｓ层的断裂呈波浪状，实质层排列不规则，局部可见瘢痕形成，Ｄｅｓｃｅｍｅｔ’ｓ层膜破裂。角膜上皮、基质和内皮层未见明显的阳性表达，Ⅴ型胶原表达在Ｂｏｗｍａｎ’ｓ层及基质层，正常及圆锥角膜中无明显差异，但在圆锥角膜瘢痕区Ⅴ型胶原表达呈强阳性。结论Ⅳ型胶原是基底膜的主要胶原，Ｂｏｗｍａｎ’ｓ层的断裂导致圆锥角膜的发病过程，最终整个过程以瘢痕形成结束。
ObjectiveTo observe the expression of collagen type Ⅳ,Ⅴ in normal cornea and keratoconus,and discuss the effects of basement membrane injury on keratoconus.MethodsImmunohistochemical SABC method was used to detect the expression of collagen type Ⅳ,Ⅴ in cornea.ResultsCollagen type IV was expressed in Bowman's layer and Descemet membrane in normal cornea and keratoconus.The positive expression decreased depending on the various corneal lesions.Bowman' membrane was wavy and intermittently broken,the lamellar layer was irregularly arranged with scars formation.Descemet'membrane was also broken.The positive expression was not seen in the epithelium,stroma and endothelium.Collagen type V was expressed in Bowman's layer and in stroma.There was no differences in normal cornea and keratoconus.The positive expression was seen in the scar tissue of keratoconus.ConclusionsCollagen type Ⅳ is the main component of basement membrane.The brokeness of Bowman's layer causes the process of keratoconus and leads to scars formation.
出处
《眼科研究》
CSCD
北大核心
2002年第1期19-22,共4页
Chinese Ophthalmic Research
