摘要
采用 ELISA 双抗体夹心法对106例 HBV 感染者共146份血清进行前 S1和前 S2抗原同步检测,结果血清前 S 抗原与血清 HBVDNA 密切相关,但并不完全一致;其与血清 HBeAg、PHSA 受体活性及抗 HBc 也具有良好的正相关性。五组不同临床类型 HBV 感染者血清前 S 抗原检出率及相对含量均以 ASC 组最高,SH 组次之,CAH、CPH 和 AH 组均较低。AH 组随疾病恢复、ALT 下降,血清前 S 抗原相对含量下降乃至阴转;CAH 和 CPH 组尽管病情改善、ALT 下降,血清前 S 抗原相对含量并无明显改变;SH 组则无论病情好转或恶化,血清前 S 抗原相对含量均下降。表明连续定量检测血清前 S 抗原可为除 SH 外其它各型乙型肝炎提示预后。作为 HBV 复制和疾病预后观察指标血清前 S1抗原较前 S2抗原敏感。
The serologic expression of both pre-S1 and pre-S2 antigens in acute and chronic HBVinfection was serially investigated by using monoclonal antibodies coated ELISA assay.Serum pre-S1 and pre-S2 antigens had been detected in 66.7% and 66.7% of acute hepa-titis B patients respectively,in 85.5% and 80.8% of CAH-B,83.3% and 79.2% ofCPH-B,91.7% and 83.3% of fulminant hepatitis,and 100% and 85.0% of asymptomaticHBsAg carriers.In acute hepatitis,pre-S1 and pre-S2 were detected at the time of maxi-mal ALT elevation,then the level of these antigens declined subsequently.The disappearance ofpre-S antigens usually implied the clearance of virus and clinical recovery.Nevertheless,pre-S antigens of chronic hepatitis remained positive throughout the period of follow-upstudy,even after serum ALT became normal.Serum pre-S antigens were highly correlated withserum HBV-DNA,HBeAg and HBV PHSA-receptor.The results of this study showed thatthe determined serum pre-S antigens could be utilized as the markers of viral replication,and they are extremely useful to monitor the process of HBV infection.
出处
《上海医学》
CAS
CSCD
北大核心
1991年第11期631-634,共4页
Shanghai Medical Journal
