摘要
目的 :观察异丙酚 (Propofol)及肿瘤坏死因子 α(TNF α)对培养的人脐静脉内皮细胞 (HUVECs)凋亡相关基因Bcl 2及Bax表达的影响 ,探讨异丙酚抑制凋亡的机理。方法 :将培养的人脐静脉内皮细胞 3～ 4代于融合状态 ,随机分为 7组 :对照组 (P0 ) ,2 5 μmol/L异丙酚组 (P2 5) ,TNF α组 (P0 +TNF α) ,12 .5 μmol/L异丙酚和TNF α组 (P12 .5+TNF α) ,2 5 μmol/L异丙酚和TNF α组 (P2 5+TNF α) ,5 0 μmol/L异丙酚和TNF α组 (P50 +TNF α) ,10 0 μmol/L异丙酚和TNF α组 (P10 0 +TNF α)。各组加入相应药物培养 2 4h后收获细胞 ,采用免疫细胞化学染色方法测定Bcl 2及Bax蛋白表达。结果 :与对照组 (P0 )相比 ,异丙酚 2 5 μmol/L组 (P2 5)Bcl 2及Bax蛋白表达无明显变化 (P >0 .0 5 ) ;TNF α(P0 +TNF α)组Bcl 2蛋白表达降低 ,Bax蛋白表达升高 (P <0 .0 0 1) ;而不同浓度的异丙酚预处理后再加入肿瘤坏死因子的各组 (P12 .5+TNF α、P2 5+TNF α、P50 +TNF α、P10 0 +TNF α)Bcl 2蛋白表达增加 ,Bax蛋白表达降低 (P <0 .0 5或P <0 .0 1)。结论 :临床相关浓度的异丙酚可通过调节Bcl 2及Bax蛋白表达抑制TNF
Abstract:To investigate the effects of propofol and TNF α in apoptosis related genes expression on cultured human umbilical vein endothelial cells(HUVECs). Methods: The third and forth passages of the cultured EC were devided into seven groups, P 0,P 25 ,P 0+TNF α,P 12.5 +TNF α,P 25 +TNF α,P 50 +TNF α,P 100 +TNF α.Treated with different concerntration of drugs for 24 hours.Bcl 2 and Bax were detected by immunocytochemical staining technique.Results:There were no significant difference between Group P 0 and Group P 25 (P>0.05). But the expression of Bcl 2 was decreased and Bax was increased in TNF+P 0 Group(P<0.001). The process could be inhibited by pre treatment with propofol(P<0.05 or P<0.01). Conclusion: Propofol in clinical relevant concentration has depressive effect on TNF induced apoptosis in HUVECs with the regulation of Bcl 2 and Bax protein expression.
出处
《武汉大学学报（医学版）》
CAS
2001年第4期334-336,346,共4页
Medical Journal of Wuhan University
