摘要
本文介绍了亚硝基胍(NTG)、溴乙锭(EB)、紫外线(UV)和吖啶类染料等各类诱变剂对含硫多肽类抗生素那西肽产生菌的诱变,其中吖啶类染料的诱变作用最强,突变率可达0.4％以上。本文也比较了发芽孢子和原生质体的诱变,结果显示了两个材料的突变率比较接近。通过对1万余株诱变后菌株的观察,本研究共获得15株负变株。3株正变株和4株营养缺陷型。这些突变株将作为进一步研究的材料和手段。
This paper reports the mutagenio effects of NTG, UV, EB and Aoridine Orange on Streptomyces actuosus which produces nosiheptide. The results showed aoridine orange to be the best, with a mutagenio rate of about 0.4%. The mutagenic effects on budding spores and protoplasts were the same. From about 11000 colonies obser-dved,we obtained 22 mutants. Of these, 15 were negative mutants. 3 mutants produced more nosiheptide than the wild type. We also obtained 4 auxotophs. These mutants will be used as implementa for further studies on the biosynthesis of nosiheptide.
出处
《上海医科大学学报》
CSCD
1991年第2期133-136,共4页
Journal of Fudan University(Medical Science)
关键词
那西肽
突变
生物合成
cosiheptide
mutation
biosynthesis
