摘要
目的 探讨ｐ２１ＷＡＦ１蛋白表达与胃癌发生、发展、浸润转移及临床分期的关系。方法 采用 Ｓ－Ｐ免疫组化法，分别检测２０例正常胃粘膜、４０例不典型增生胃粘膜和１０８例胃癌ｐ２１ＷＡＦ１蛋白表达。结果ｐ２１ＷＡＦ１阳性表达率：正常与不典型增生胃粘膜分别为 １００％、９２％，显著高于胃癌 ３９％（Ｐ ＜０．０５）；高分化腺癌６３％，分别与低分化腺癌３５％、未分化癌２６％、粘液癌３０％及印戒细胞癌２０％比较，皆具有显著性差异（Ｐ＜０．０５）；临床Ⅰ期６０％和Ⅱ期５６％显著高于Ⅲ期２７％和Ⅳ期２２％（Ｐ＜０．０５）；伴淋巴结转移３５％与未转移６２％有显著差异（Ｐ＜ ０．０５）；浸润浅肌层 ６０％较浸润深肌层 ３５％差异有显著性意义（Ｐ＜ ０．０５）而与性别、年龄无关。结论ｐ２１ＷＡＦ１蛋白失表达与胃癌发生、分化程度、浸润、淋巴结转移及临床分期相关。
Objective To study the relationship between expression of p21WAF1 gene and carcinpogenesis, invasion and metastasis. Methods Expression of p21WAF1 protein was respectively examined by streptavidin- peroxidase conjugated method (SP) in normal gastric mucosa, dysplastic gastric mucosa and gastric carci- noma. Results The expression of p2lWAFi in 100% (20/20) of normal gastric mucosa and 92%(37/40) of dysplastic gastric mucosa was markedly higher than that in 39%(43/108) of gastric carcinoma (P<0. 05). The positive frequency of p21WAF1 in 6300 (19/30)of well-differentiated was significantly higher than that in 35%(14/40) of poorly-differentiated , 26% (4/15)of undifferentiated , 30% (4/13) of mucoid carcinoma and 20%(2/10) of singnet ring cell carcinoma (P< 0.05). A significant relationship was found between p2LWAF1 expression and depth of invasion (P < 0.05). The p21WAF1 positively in 35% of 20 pairs of samples from primary carcinoma and their lymph node metastases was lower than that in 62%(25/60) of carcinoma without lymph node metastases (P< 0. 05). The positive expression of p21WAFI was 60% (9/15), 56% (18/32)and 22% (4/18)in clinical stageⅠ、 Ⅱ、Ⅲ 、 and Ⅳ , respectively , the positive difference of p21WAF1 were significant between Ⅰ、 Ⅱ and Ⅳ (P<0. 05) . There was rio association between p21WAF1 expression and sex, age. Conclusion It is indicated that loss of p2lWAF1 protein expression is related to carciongenesis, degree of differentiation, depth of invasion, lymph node metastasis and clinical stage in gastric carcinoma.
出处
《肿瘤防治研究》
CAS
CSCD
2002年第1期43-45,共3页
Cancer Research on Prevention and Treatment
基金
湖南省卫生厅"8.5"重点攻关项目(No.9301)
