摘要
应用端粒酶聚合酶链反应 (PCR) -酶联免疫吸附测定 (ELISA)法 ,检测手术切除标本中肺癌组织、癌旁组织及正常肺组织端粒酶活性。结果显示 :1 5例肺癌组织端粒酶活性为 0 84± 0 3 8;其中 8例鳞癌为 0 82± 0 3 9,7例腺癌为 0 86± 0 3 9,明显高于 1 5例癌旁组织 0 1 6± 0 0 3和 3例正常肺组织 0 1 4± 0 0 2 (均P <0 .0 0 1 )。以吸光度A450>0 .2 0为阳性 ,1 5例肺癌组织端粒酶活性阳性率为 86 7% (1 3 /1 5 ) ;其中腺癌阳性率为 85 7% (6 /7) ,鳞癌阳性率为87 5 % (7/8) ;而 1 5例癌旁组织和 3例正常肺组织端粒酶活性表达均为阴性。提示端粒酶活性可作为非小细胞肺癌诊断的特异性较强的分子标记物 ;TRAP PCR ELISA法检测端粒酶活性具有方法简便 ,结果可靠。
Objective To investigate the telomerase activity in human non small cell lung cancer (NSCLC) and to investigate the possibility of telomerase as a tumor biological marker. Methods The semi-quantitative assay and expression of PCR based telomeric repeats amplification Protocol ELISA(TRAP PCR ELISA) and PAGE (TRAP PCR PAGE) were detected in 15 NSCLC tissues and 15 tumor adjacent tissues, 3 normal lung tissues. Results No telomerase activity in 15 tumor adjacent tissues and 3 normal lung tissues was detected, However, of 15 NSCLC tissues, 13 were positive for telomerase activity with a 86.7% positive rate, telomerase activity was significantly higher in NSCLC than in Tumor adjacent tissues and normal lung tissues (P<0.001). Conclusion The results suggest that telomerase is a tumor specific gene marker.
出处
《湖南医科大学学报》
CSCD
北大核心
2001年第6期549-550,共2页
Bulletin of Hunan Medical University
关键词
肺肿瘤
癌
非小细胞性
端粒酶
lung neoplasma
carcinoma, non small cell
telomerase
