摘要
采用PCR -RFLP对南昌白猪 (117头 )和大约克夏猪 (36 1头 )的GH 2基因 - 119～ +486bp的片段进行了扩增 ,并用ApaⅠ酶切 ,产生了 2个等位基因A(44 9+10 1+5 5bp)和B(316 +133+10 1+5 5bp)。分析了不同基因型对个体初生重、2月龄重、4月龄重、6月龄重、校正背膘厚、平均背膘厚、瘦肉率和料重比等生产性能的影响。结果表明 ,在南昌白猪中 ,不同GH 2基因型间在所测的生产性能上差异不显著 (P >0 0 5 ) ;在大约克夏猪中 ,AA型猪的瘦肉率最低、与BB型猪相比 ,差异显著 (P <0 0 5 )。
The genotypes of 117 Nanchang White pigs and 361 Large Yorkshire pigs at GH 2 locus were detected by PCR RFLP. The PCR products were cut by Apa Ⅰ, and produced two alleles: A (449+101+55bp), and B (316+133+101+49bp). Effects of different genotypes on some important production traits involving the birth weight, 2 month body weight, 6 month body weight, corrected back fat thickness, average back fat thickness, feed to gain ratio and lean percentage were analyzed. The results showed that in Nanchang White pigs, no significant differences were observed between different genotypes and different growth and carcass traits; while in Large Yorkshire, the pigs with BB genotype had more lean percentage than pigs with AA genotype ( P <0 05).
基金
国家"973"项目 (G2 0 0 0 0 1610 0 )
国家自然科学基金项目 (3 0 0 60 0 5 8)资助~~
