摘要
以质膜 Ca2 +通道阻断剂 L a Cl3和钙调素 (Ca M)拮抗剂氯丙嗪 (CPZ)对水稻幼苗预处理以阻碍 Ca2 +· Ca M信使传导后 ,研究了盐胁迫下 ,稻苗保护酶活性的变化。结果表明 :0 .15 mol/ L Na Cl胁迫下 ,L a Cl3和 CPZ预处理显著加剧稻苗过氧化氢酶 (CAT)活性和抗坏血酸过氧化物酶 (APX)活性下降 ;对谷胱甘肽还原酶 (GR)活性则无明显影响 ;而超氧化物岐化酶 (SOD)和过氧化物酶 (POD)活性前期无明显变化 ,仅在胁迫后期明显下降。盐胁迫下 ,L a Cl3和 CPZ预处理加剧 SOD、 CAT和 APX活性下降与加剧稻苗 MDA含量积累呈显著或极显著正相关。这暗示 Ca2 + · Ca
After being blocked Ca 2+ ·CaM messenger transduction by pretreating with LaCl 3 or CPZ (chlorpromazine), activities of antioxidant enzymes in rice seedlings under salt 0.15 mol/L NaCl stress were investigated. The results showed that under salt stress, pretreatment led to more serious decrease in activities of ascorbate peroxidase (APX), catalase (CAT) and superoxide dismutase (SOD), whereas glutathione redutase (GR) had no significant change during stress, and peroxidase (POD) had little change at the early stage, then decreased significantly at the late stage of stress. The enhanced increase value of MDA content was significantly positively correlated with the enhanced decrease value in activities of CAT, APX and SOD by pretreatment of LaCl 3 and CPZ under salt stress. It is suggested that the effects of LaCl 3 and CPZ on stress resistance of rice seedlings were mainly related to SOD, CAT and APX under salt stress. The results also suggested that Ca 2+ ·CaM messenger system might regulate the stress resistance of rice seedlings by affecting activities of some antioxidant enzymes.
出处
《作物学报》
CAS
CSCD
北大核心
2002年第1期42-46,共5页
Acta Agronomica Sinica
基金
广东省自然科学基金 (990 0 2 4)
华南农业大学校长基金 (990 0 4)资助
