摘要
目的 观察血链菌生物膜形成初期的死菌、活菌粘附情况 ,并对其粘附能力进行比较。方法 血链菌混悬于人工唾液 ,其中活菌百分比为 70 %,在模仿体内环境的人工口腔内 ,血链菌悬液以 0 .8ml/min均速滴至唾液覆盖的玻片以形成生物膜 ,应用荧光染色技术观察血链菌生物膜形成中死 /活细菌的初期粘附 ,计算活菌百分比 ;并通过疏水实验、红细胞凝集实验对死 /活菌的粘附特性进行比较。结果 血链菌生物膜形成的 30min ,6 0min ,12 0min ,2 40min ,其中活菌百分比分别为 30 %,5 0 %,6 0 %,70 %;在同一时间血链菌悬液的活菌百分比几乎是恒定的 ,两者相比 :在 30min ,6 0min具有显著性差异 (P <0 .0 5 ) ;在 12 0min ,2 40min无显著性差异 (P>0 .0 5 )。疏水实验、红细胞凝集实验中 ,死菌的疏水性能、红血球凝集能力均强于活菌。结论 血链菌生物膜形成的初期 ,死菌可能比活菌更易粘附于固体表面。
Objective To determine the role of dead/live microorganisms during the initial events of biofilm formation and their adherent characteristics.Methods Vital fluorescent staining technique was used to observe the dead/live adherent microorganisms on the saliva coated glass (SCG) in an artificial mouth model.The adherent ability of dead/live cells were determined by hemagglutination and hydrophobicity experiments.Results During streptococcus sanguis biofilm formation,the proportion of vital cells in the biofilm was significantly lower than that in the suspension at 30,60 min (P< 0.05 ),whereas the proportion of vital cells was not different between biofilm and suspension at 120,240 min (P> 0.05 ).Furthermore,the hydrophobicity and bemagglutination ability of dead cells were stronger than live cells.Conclusion During the initial events of biofilm formation,the dead cells rather than vital cells attach preferably to solid surface.
出处
《现代口腔医学杂志》
CAS
CSCD
2002年第1期1-3,共3页
Journal of Modern Stomatology
关键词
死菌
活菌
生物膜
疏水性
红血球凝集
Dead/live cells Biofilm formation Hydrophobicity Hemagglutination
