摘要
用 CTAB法从枯草杆菌 (N atto)提取基因组 DNA,聚合酶链式反应 (PCR)扩增获得约 1.3kp的 DNA片段 ,在 T4DNA连接酶作用下 ,将扩增片段连接到 p UCm- T载体上 ,转化 Ecoli DH5 a感受态细菌。将初步鉴定的阳性细菌提取质粒 DNA进行 PCR鉴定和测序 。
Bacillus subtilis(natto) genomic DNA was prepared with CTAB method.The purpose gene were gotten by PCR,then was ligated into pUCm-T vector,transformed it into E.coli DH 5 α competent cell.The positive plasmid were extracted,PCR amplification and sequence analysis demonstrated that the nattokinase gene including all of ORF(Opening Reading Fragment) was gotten.
出处
《内蒙古农业大学学报（自然科学版）》
CAS
2001年第3期31-34,共4页
Journal of Inner Mongolia Agricultural University(Natural Science Edition)
基金
国家自然科学基金项目 (39860 0 4 0 )
