摘要
实验用鲫平均体重 10 0 g ,采用免疫物质被动转移试验 ,探讨体液免疫在鲫感染温和气单胞菌(Aeromonassobria)中的保护作用。结果表明 ,无论免疫物质是来自异源还是自源 ,是由相关的嗜水气单胞菌 (A .hydrophia)诱导产生还是由攻毒的温和气单胞菌直接刺激 ,均具有良好的免疫保护作用。实验还发现 ,再次攻毒时 ,在所有抗体组的鲫存活率为 10 0 %时 ,全血被动转移组的死亡率高达 5 5 .6 % ;免疫前后免疫复合物发生明显变化。
Passive immunization of Crucian carp was conducted to evaluate the role of immune protection when the C.carp was infected with Aeromonas sobria. The immune substances came from heterogeneous source such as chicken immune antibody, or self antibody, or the antibodies induced from Aeromonas hydrophia or by stimulating the challenging bacterium (A.Sobria) directly. The results show that the protection effects above are all better than the control (unimmune C.carp injected with A.sobria). But the rechallenge test shows that all the immune antibody groups have 100% survival rate except the whole fish blood immune group which has 55.6% mortality. Moreover,the immune complexes changes largely compared with pre immune sera. It comes to the conclusion that the antibody plays a key role in protecting C. carp from fatal infection by A.sobria. It's also verified that after A.hydrophia immune, C.carp can be protected from A.sobria, which implies that research of immune cross protection is very significant for the invention of new vaccine.
出处
《中国水产科学》
CAS
CSCD
北大核心
2001年第4期41-45,共5页
Journal of Fishery Sciences of China
基金
国家自然科学基金资助项目 (3 9770 5 85 )
IFS基金资助项目 (A/2 3 3 8-2 )
关键词
温和气单胞菌
嗜水气单胞菌
鲫
抗体
被动免疫
Aeromonas sobria
Aeromonas hydrophia
Crucian carp
antibody
passive immune
