摘要
以氨基末端磁微粒为载体 ,用戊二醛作交联剂 ,通过共价交联结合法固定化AS1 398中性蛋白酶 .可以制备出活力达 45 0 0 0U/ g磁性固定化酶 .探讨了该载体对中性蛋白酶的最适固定化条件 ,并对磁性固定化酶的热稳定性 ,储存稳定性、操作稳定性等进行了研究 ,确定了此载体对酶的固载能力大于 2 0 0mg/ g (载体 ) ,及固定化磁性酶最适 pH为 7 5 ,最适温度为 6
Neutral proteinase was covalently attached to amine terminaled magnetic particles and cross linking it with glutaraldehyde. Activity of MIE arrive 45 000 U/g (magnetic particles). Optimum conditions of immobilization were studied. Stabilities to heat and preservation,operational stability of free enzyme and MIE also were compared. Some properties of MIE, such as optimum pH is 7.5, optimum temperature is 60℃ were confirmed.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
2001年第5期691-694,共4页
Progress In Biochemistry and Biophysics
关键词
氨基末端磁微粒
中性蛋白酶
戊二醛
固定化磁性酶
磁性载体
amine terminaled magnetic particle, neutral proteinase, glutaraldehyde, magnetic immobilized enzyme
