摘要
运用离子对色谱法 ,以 (+) 10 樟脑磺酸为手性离子对试剂 ,在二醇柱 (Lichrospher 10 0 DIOL)上 ,分离了苯丙醇胺、美托洛尔、普萘洛尔、肾上腺素和沙丁胺醇 5种氨基醇类对映体。考察了流动相组成和化合物结构对分离选择性的影响。选用的流动相为二氯甲烷 正戊醇 (97 3、V V、内含 2 .2× 10 - 3mol L的樟脑磺酸 ) ,流速为 0 .3mL min。苯丙醇胺、美托洛尔、普萘洛尔、肾上腺素和沙丁胺醇对映体的分离因子分别为 1.2 9、1.2 5、1.2 5、1.33和 1.2 9;它们的分离度分别为 1.5 5、1.78、1.83、1.89和 1.
Enantiomers of five amino alcohols were resolved by ion pair chromatography on a Lichrospher 100-DIOL column with (+)-10-camphorsulphonic acid as the chiral counter ion. Studies of the influence of the mobile phase composition and the solute structure on separation factors of solutes are presented. The chosen mobile phase is dichloromethane/1-pentanol (97/3, V/V, containing 2.2 x 10(-3) mol/L (+)-10-camphorsulphonic acid). For norephedrine, metropolol, propranolol, adrenaline and salbutamol, the separation factors are 1.29, 1.25, 1.25, 1.33 and 1.29, and the resolutions are 1.55, 1.78, 1.83, 1.89 and 1.78 respectively.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2001年第11期1295-1298,共4页
Chinese Journal of Analytical Chemistry
