摘要
目的 :探讨姜黄素对 HL- 6 0细胞周期的影响。方法 :选取 HL- 6 0细胞系 ,作细胞培养 ,采用 SABC法测定分化抗原 CD1 1 a和 CD1 4 阳性率 ,用 TU NEL 法判定细胞凋亡 ,用 FCM法分析细胞周期 ,检测 p2 7kip1、p2 1waf1、cyclin D3及 p Rb P-表达率。结果 :姜黄素呈时间及剂量依赖性抑制 HL- 6 0细胞增殖 ,用 5、10、2 5 μmol/ L 作用 18h,增殖抑制率为 4.7± 1.2 %、18.0± 2 .0 %和 37.5± 2 .4% ;2 4h后抑制率达 6 .3± 1.9%、2 5 .0± 2 .2 %和 43.8±2 .0 % ,可增加 p2 7kip1、p2 1waf1表达 ,降低 cyclin D3表达 ,使去磷酸化 p Rb(p Rb P- )表达量增高 ;用 10 μmol/ L 姜黄素作用 4d,p2 7kip1 、p2 1waf1 、p Rb P- 阳性率为 47.8± 3.6 %、42 .7± 2 .2 %和 2 3.5± 1.5 % ,FCM进一步发现姜黄素作用后 ,p2 7kip1 、cyclin D3荧光强度分别较对照组增高 7倍及降低 74.93%。细胞周期被阻滞于 G0 / G1 和 G2 / M期 ,进而走向凋亡 ,且此能力呈时间及剂量依赖性。结论 :姜黄素能干扰细胞周期进程 ,诱导 HL - 6 0细胞凋亡 ,其作用机制与上调 p2 7kip1 、p2 1waf1 及下调 Cyclin D3蛋白表达 ,增加 p Rb P- 表达水平 ,调控 G1 / S和 G2 / M期检测点功能有关。
Objective:To explore the effects of curcumin on cell cycle in HL 60 cell line. Method:Cellular culture, NBT reduction test, SABC, TUNEL and flow cytometry were used. Result:Curcumin selectively inhibite proliferation of HL 60 cell line in a dose and time dependent manner. HL 60 cell treated by curcumin at 5,10,25 μM for 18 hours, the proliferate inhibition rate reached 4.7 ± 1.2 %,18± 2.0 %, 37.5 ± 2.4 %; 24 hours, the proliferate inhibition rate was 6.25 ± 1.9 %,25± 2.2 %, 43.75 ± 2.0 % respectively. The expressions of p27 kip1 , p21 waf1 , and pRb P- were elevated in the presence of curcumin. However, the level of cyclinD3 was decreased under the effect of curcumin. The positive rates of p27 kip1 , p21 waf1 , and pRb P- were 47.83 ± 3.6 %, 42.69 ± 2.2 %, 23.52 ± 1.5 %. Cell cycle was arrested in G 0/G 1 and G 2/M phase followed by inducing cell apoptosis .Conclusion:Curcumin could disturb cell cycle progression of HL 60 and lead to apoptosis, thus selectively inhibite proliferation of HL 60 cell line in a dose and time dependent manner. The mechanism appeared to be associated with regulation of G 0/G 1 and G 2/M checkpoints, up regulation of p27 kip1 , p21 waf1 and pRb P- , whereas down regulation of cyclin D3.
出处
《临床血液学杂志》
CAS
2001年第5期215-217,共3页
Journal of Clinical Hematology
