摘要
以转 Bt基因抗虫棉 R55为材料 ,利用ELISA( Enzyme-linked Immunosorbent Assay)检测方法 ,通过对不同亲本与抗虫亲本 R55杂交F1～ F5、BC1、BC2 世代材料 Bt晶体杀虫蛋白的定性、定量测定 ,结合大田自然感虫条件下的抗棉铃虫鉴定 ,研究了转 Bt基因抗虫棉 Bt基因的遗传规律。结果表明 :F1均表现阳性 ,F2 阳、阴性株符合 3∶ 1的分离比例 ,回交 BC1阳、阴性株呈现 1∶ 1的分离比例 ,说明 Bt基因的遗传基本符合显性主基因遗传规律。但 Bt基因的遗传又有其特殊性 ,表现在亲本的遗传背景对 Bt基因的表达有着较大影响 ,不同亲本与抗虫亲本杂交 F1代 Bt晶体蛋白的表达量存在较大差异 ;杂交方式对 Bt基因的表达亦有一定影响 ;不加选择的连续回交 ,有可能使 Bt基因“丢失”。未发现 Bt基因的表达随世代的递增、农艺和经济性状的改进而降低的趋势 。
The inheritance of Bt gene in Bt transgenic cotton has b een studied b y determining the Bt crystal toxin protein of the populations, strains and lines from F 1 to F 5 and BC 1,BC 2 generations derived from the crosses of tran sgenic cotton line R55 with a lot of normal parents. ELISA(Enzyme-linked Immuno sorbent Assay) method was used for the determination of Bt crystal protein and c ompared with the biological identification in the field. The results showed that the inheritance of the Bt gene accorded basically with the inheritance law of a major dominant gene. But it also showed its own particularity. The background o f the normal parents greatly affected the expression of the Bt gene. Both amount of the toxin protein in F 1 plant and ratio of positive plants in F 2 populat ion varied with crosses of different normal parents and R55; The style of cross also affeded the expression of the Bt gene; Successive back cross without selec tion for boll worm resistance could cause the Bt gene to be 'lost'. The expre ssion of the Bt gene did not weaken in the following generations after cross, wh ile agronomic and enconomic characters have been improved. The Bt gene could be stably inherited.
出处
《棉花学报》
CSCD
北大核心
2001年第5期268-272,共5页
Cotton Science
基金
"国家转基因植物研究与产业化专项"资助
关键词
转BT基因棉
抗虫性
遗传
抗虫棉
棉花
transgenic Bt gene cotton
bollworm resistance
inh eritance
