摘要
采用UV光谱法 ,发现在pH 7～ 8的缓冲溶液中 ,脱氧核糖核酸 (DNA)与 3 氨基 6 二甲氨基 2 甲基吩嗪盐酸盐 (ZR)相互作用 ,生成红色配合物 ,此配合物的最大吸收峰为 5 2 0nm。反应前后的吸收光谱变化明显 ,配合物的吸收峰值红移 70nm。测得该配合物的表观摩尔吸光系数ε =1 5× 10 6mol-1·L·cm-1;最大结合数n =30 3。研究了体系的酸度、温度、时间等基本反应条件。离子强度的改变对体系的吸光度有明显的影响。实验研究了生物大分子与小分子探针的结合模式 ,认为该反应基本符合Scatchard模型。
The interaction of deoxyribonucleic acid (DNA) and 3 amino 6 dimethylamino 2 methlphenazine hydrochloride (ZR) was investigated by UV spectrophotometric method in solution (pH 7 4). When DNA was added into ZR solution, the red color was observed, which indicated formation of the DNA ZR complex. The maximum absorption of the complex was 520 nm with the apparent molar absorptivity of ε =1 5×10 6 mol -1 ·L·cm -1 .The maximum absorption of the complex was shifted 70 nm than with ZR. The maximum binding number is n =303. The basic reaction conditions were investigated. It is found that sodium chloride concentration of the solution has significant effect on the sensitivity of DNA ZR complex. The Scatchard model is appropriate in the treatment of data obtained here.
出处
《光谱学与光谱分析》
SCIE
EI
CAS
CSCD
北大核心
2001年第6期814-818,共5页
Spectroscopy and Spectral Analysis
基金
国家自然科学基金 (基金号 40 0 72 0 2 0 )
四川省教委
科委基金资助
