摘要
建立了先用体积分数为 80 %的甲醇水溶液回流提取烟草样品中的植物多酚 ,再采用Sep Pak C18固相微萃取小柱预分离脱脂的前处理方法 ,并选择了合适的分析烟草样品中植物多酚的高效液相色谱条件。采用C18柱 ,甲醇 磷酸二氢钾缓冲液为流动相进行梯度洗脱 ,烟草中主要的植物多酚均达到基线分离。提取各组分在其最大波长下的色谱图 ,根据其峰面积定量 ,并用紫外光谱图对烟草中主要的植物多酚进行辅助定性。在该方法中 ,多酚的标准回收率为 94%～ 10 5 %,RSD为 1 2 8%～ 1 49%。
A high performance liquid chromatographic (HPLC) method for the determination of polyphenols in tobacco was studied Polyphenols can be extracted from tobacco sample by refluxing with 80% (volume fraction) methanol, then subjected to degrease by solid phase extraction with Sep Pak C 18 column Chlorogenic acid, rutin, scopoletin, caffeic acid, scopolin and other polyphenols can be separated on a Nova Pak C 18 column (3 9 mm i.d.×150 mm) gradiently eluted with methanol and 0 05 mol·L -1 potassium dihydrogen phosphate buffer solution as mobile phase at a flow rate of 0 5 mL·min -1 Polyphenols were determined at the maximum wavelength of each polyphenol, for chlorogenic acid 326 1 nm, rutin 354 8 nm, scopoletin 344 0 nm, caffeic acid 323 7 nm and scopolin 365 2 nm by photodiode array detector The main polyphenols in tobacco can be identified with UV spectroscopy The recoveries of tobacco polyphenols were 94% 105%, and the relative standard deviations were 1 28% 1 49% This method can be applied to the determination of polyphenols in tobacco with satisfactory results
出处
《色谱》
CAS
CSCD
北大核心
2001年第6期564-566,共3页
Chinese Journal of Chromatography
基金
云南省烟草公司重点资助项目 ( 2 0 0 0B2 0 1)
关键词
高效液相色谱
固相萃取
植物多酚
烟草
测定
分析
high performance liquid chromatography
solid phase extraction
plant polyphenol
tobacco
