摘要
目的 探讨血浆纤溶酶原激活物抑制物 - 1(PAI- 1)基因启动子区 4G/5 G多态性与中国北方汉族人 2型糖尿病合并肾病的相关性。方法 运用等位基因特异性引物 PCR扩增技术 ,检测病程超过 10年的 2型糖尿病患者 (143例 )和健康人 (85例 )的 PAI- 1基因 4G/5 G多态位点的基因型 ,用发色底物法测血浆 PAI- 1活性。结果 (1)糖尿病肾病组血浆 PAI- 1活性明显高于糖尿病非肾病组 (P<0 .0 5 )。 (2 ) 4 G/4 G基因型者 PAI- 1活性明显高于 4G/5 G和 5 G/5 G者 (P<0 .0 0 5 )。 (3)糖尿病肾病组 PAI- 14G/4 G基因型频率 (39% )和 4G等位基因频率 (6 0 .5 % )高于糖尿病非肾病组 (33%和 5 6 .5 % ) ,但无统计学意义 (P>0 .0 5 )。结论 PAI- 1活性升高是糖尿病肾病的独立危险因素 ,PAI- 1基因启动子区4G/5 G多态性与 PAI- 1活性密切相关 ,4G/4 G基因型可能是易发糖尿病肾病的遗传标记。
Objective To investigate the association of the PAI 1 promoter 4G/5G polymorphism and diabetic nephropathy in type 2 diabetes mellitus of North China.Methods The 4G/5G allele polymorphism in the PAI 1 gene promoter region were genetyped by allele specific PCR in 143 type 2 diabetic patients who had diabetes for over 10 years and 85 normal controls.The activity of PAI 1 was assayed by a chromogenic substrate method.Results Patients with type 2 diabetic nephropathy had a higher frequency of PAI 1 4G/4G genotype (39%) and 4G allele (60.5%) than those without nephropathy (33% and 56.5%),though not significantly ( P >0.05).The plasma PAI 1 activity in the diabeties with nephropathy was higher than that in the subjects without nephropathy ( P <0.05).PAI 1 activity was significantly higher in 4G allele homozygous than those in 4G/5G heterozygous and 5G homozygous ( P <0.005).Conclusion Elevated plasma PAI 1 activity is a risk factor for diabetic nephropathy.The presence of the 4G allele of PAI 1 gene was associated with a higher level of PAI 1 activity,and may involved in the generation of diabetic nephropathy.
出处
《中国糖尿病杂志》
CAS
CSCD
2001年第6期333-336,340,共5页
Chinese Journal of Diabetes
