摘要
目的探讨术前区域动脉灌注化疗对血管内皮生长因子(VEGF)蛋白表达及肿瘤血管生成的影响。方法通过免疫组织化学方法,检测术前区域动脉灌注化疗及根治手术的结直肠癌病例癌组织中微血管密度(MVD)和VEGF蛋白表达水平,并与同期未行术前动脉化疗而直接手术的31例病例作比较。结果术前动脉化疗组的MVD值为19.2±5.2,而对照组为42.4±18.4,两组间差异有显著性意义(P<0.01)。术前动脉化疗组VEGF表达亦明显低于非化疗组(P<0.01)。术前化疗组肿瘤的MVD与VEGF表达相关分析表明两者间存在正相关(r=0.788,P<0.05),VEGF的表达与肿瘤Dukes分期相关且有统计学意义。结论结直肠癌术前区域动脉化疗可以通过抑制肿瘤细胞分泌VEGF蛋白,或直接作用于肿瘤血管,减少肿瘤的血管生成,减少肿瘤细胞的血供,从而改善结直肠癌患者的治疗效果和预后。
Objective To explore the effect of preoperatively regional intraarterial infusion chemotherapy(PRAC) on the microvessel density (MVD) and the expression of vascular endothelial growth factor (VEGF) of tissures from colorectal cancer. Methods The MVD and the expression of VEGF were examined by immunohistochemistry method in cancerous tissues from 30 patients with PRAC and from 31 patients without PRAC. Results Mean MVD was 19.2±5.2 in the chemotherapeutic group, and 42.4±18.4 in the control group(P< 0.01).Expression of VEGF protein was remarkably lower in chemotherapeutic group than that in the control group (P< 0.01) . The expression of VEGF was correlated with MVD(r=0.788,P< 0.05) . Conclusion PRAC may take effect by down regulating the expression of VEGF to reduce tumor angiogenesis or directly inhibiting tumor angiogenesis to inhibit the proliferation of cancer cells.
出处
《中华胃肠外科杂志》
CAS
2001年第4期218-221,共4页
Chinese Journal of Gastrointestinal Surgery
基金
卫生部优秀青年科技人才专项科研基金(97061)
关键词
结直肠肿瘤
区域性化疗
微血管密度
血管内皮生长因子
Colorectal neoplasm
Regional chemotherapy
Microvessel density
Vascular endothelial growth factor
