摘要
目的 :探讨牛肺动脉内皮细胞 (BPAEC)产生过氧亚硝基阴离子 (ONOO )的能力及其作用。方法 :用流式细胞免疫荧光技术 ,定量检测内毒素主要成分脂多糖 (LPS)诱导培养的BPAEC中ONOO-生成标志物硝基酪氨酸 (NT)的含量 ,观察ONOO-对BPAEC形态变化的影响。结果 :LPS可剂量依赖性诱导BPAEC产生ONOO-明显增多 ,并为氨基胍部分翻转 ;ONOO-可导致BPAEC明显回缩 ,胞体变小 ,细胞间隙增宽。结论
Aim: To explore the significance of bovine pulmonary arterial endothelial cell (BPAEC) to produce peroxynitrite (ONOO -). Methods: The contents of nitrotyrosine (NT),a marker of ONOO - generation in vivo ,were quantitatively measured with flow cytometric and immunofluorescent technique in cultured BPAEC induced by lipopolysaccharide(LPS),a major component of bacterial endotoxin. In addition,effect of ONOO - on micromorphologic features of BPAEC was observed. Results: LPS caused significant increase of NT in BPAEC in a dose dependent manner,which was in part reversed by aminoguanidine,a selective inhibitor of inducible nitric oxide synthase. Exposure of BPAEC to exogenous ONOO - resulted in the cellular retro contraction with diminshed cellular body and increased width of cellular junction. Conclusion: LPS may induce the ONOO - formation of BPAEC. Endothelial cell derived ONOO - may mediate the deleterious effect of LPS on BPAEC per se .
出处
《中国应用生理学杂志》
CAS
CSCD
北大核心
2001年第4期379-382,共4页
Chinese Journal of Applied Physiology
基金
国家自然科学基金资助课题 ( 39870 317)
