摘要
用建立非表达文库的方法 ,从龙角性状家蚕中肠组织的mRNA合成cDNA片段 ,重组入噬菌体λgt10的EcoRI的位点之间 ,所构成的基因非表达文库库容量为 1.3× 10 6,经大肠杆菌C60 0与C60 0hlf菌株平皿测定 ,重组率为 70 %。
cDNA fragments were obtained by in vitro transcription of the mRNA isolated from the mid-intestine in silkworm (Bombyx mori L.). After EcoR I adaptor ligation, the cDNA was directly ligased with the EcoR I arms of λvector, followed by Lamda DNA packaging and infected by the host bacterial strain C600hlf. According to phage plaques bright selection, the percentage of recombinant phages was 70%, while the cloning efficiency was high too, and their titer was 1.3x106 pfu/μg.
出处
《蚕学通讯》
2001年第3期1-4,共4页
Newsletter of Sericultural Science
基金
国家自然科学基金重点资助项目 (编号 390 730 370 )
博士点专项基金项目 (编号 96 0 6 0 2 )资助
关键词
家蚕
CDNA文库
中肠组织
龙角蚕
silkworm (Bombyx mori L.) mid-intestine cDNA library