摘要
目的 :选择云南红豆杉高紫杉醇含量细胞系。方法 :根据色泽、质地、生长速率和分泌有色物质等性状 ,将不同的小细胞团挑出、单独培养 ,并对其进行生长分析和紫杉醇含量测定。结果与结论 :通过仔细选择 ,可以得到紫杉醇含量高的细胞系 ;颜色深、生长慢、干重 /鲜重比值高的细胞系紫杉醇含量常常较高。
Objective: To select high taxol content cell lines of Taxus yunnanensis . Method: Choosing different cell aggregates according to their color, texture, growth rate and secretion of colorful substances, culturing them separately, and further analyzing their growth rates and taxolcontents. Result and Conclusion: Cell lines with higher taxol contents could be obtained by careful selection; those with darker color, lower growth rate and higher ratio of dry cell weight vs. fresh cell weight usually has higher taxol contents.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2001年第11期739-741,共3页
China Journal of Chinese Materia Medica
基金
湖北省教育厅资助课题 ( 98B0 0 4)
"86 3"计划( 86 3 10 2 12 0 6 0 1)
国家"九五"重点攻关项目 ( 96 C0 2 0 3 0 1)
湖北省生物化学与分子生物学重点学科基金
