摘要
目的 采用客观又简单的软琼脂细胞集落形成率的指标来探索二羟环氧苯并芘诱导 16 HBE细胞转化的理想实验方案 ,构建恶性转化的细胞模型。方法 根据细胞存活率试验及克隆形成率试验确定诱导的剂量 ,以不同剂量二羟环氧苯并芘 1次或多次处理细胞 ,观察培养的不同阶段转化灶出现情况 ,用软琼脂培养鉴定其锚着依存性生长的恶性特征 ,比较各组转化细胞的集落形成率。结果 以 0 .1、0 .5、1.0、2 .0 μmol/ L的剂量 ,对细胞多次间断染毒 ,传代 15次 ,是二羟环氧苯并芘诱导 16 HBE细胞恶性转化的理想方案 ,其集落形成率分别为 2 .0‰、5 .5‰、7.0‰、10 .5‰ ,剂量 -反应关系呈现良好的直线相关 ,r=0 .9741,P<0 .0 5。结论 二羟环氧苯并芘能诱导人支气管上皮细胞 16 HBE转化 。
Objective To findout a good method for malignant transformation of human bronchial epithelial cells 16HBE cell induced by anti benzo(a)pyrene trans 7,8 dihydrodiol 9,10 epoxide (anti BPDE) using the objective and simple index∶colony forming frequency of cells in semisolid agar culture medium,and to establish a best model of malignant transformation of 16HBE cells. Methods The tests on viable rate and colony formation of 16HBE cells were carried out to determine the exposure doses of anti BPDE.The 16HBE cells were treated once or several times by anti BPDE at different doses and the transformed foci were observed and assessed at the different stages during the period of the whole experiment.The malignant features of transformed 16HBE cells with a good dose response relationship were identified by the method of semisolid agar culture.The anti BPDE induced colony forming frequencies of 16HBE cells in semisolid agar medium in each dose group were statistically compared Results The best method for malignant transformation of 16HBE cells induced by anti BPDE was that the 16HBE cells were intermittently treated several times with anti BPDE at doses of 0 1,0 5,1 0 and 2 0 μmol/L respectively and were inoculated for 15 generations,the related colony forming frequencies were 2.0‰,5.5‰,7.0‰ and 10.5‰ respectively with a significant dose response relationship and a good linear correlation ( r= 0.9741, P<0 05 ) Conclusion The research infered that the malignant transformation of human bronchial epithelial cell 16HBE cells could be induced successfully by anti BPDE,a suitable model could be established by anti BPDE also
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2001年第3期137-139,178,共4页
Journal of Environment and Health
基金
国家自然科学基金 (39970 630
