摘要
目的 通过大鼠胸主动脉腹腔移植模型 ,进一步探讨移植物动脉硬化形成过程中平滑肌细胞增殖的机理。方法 健康纯系Wistar大白鼠为供者 ,SD大鼠为受者 ,均为雄性 ,体重 12 0～ 35 0g。共分为 3组。A组 :急性排斥组 ,共 90只 ,分别于术后 2 ,4,8周处死。每小组各 30只。B组 :慢性排斥组 ,共72只 ,分别于术后 4,8,12周处死。每小组各 2 4只。C组 :同系对照组 ,供受者均为Wistar大白鼠 ,共 72例。建立同种异体大鼠胸主动脉腹腔移植模型。采用HE染色观察血管排斥程度 ;Masson三色染色法判断动脉硬化程度 ;免疫组化染色观察α actin表达的阳性细胞数 ,以及这些阳性细胞表达PCNA、cdk1/cyclinB1、cdk2 /cyclinE、cdk4/cyclinD1的表达程度。 结果 A组整个血管壁均有明显的炎性细胞浸润 ,外膜病变比内膜更重。外膜α actin阳性细胞数随着时间推移、病程进展明显增多。这些α actin阳性细胞高度表达PCNA、cdk1/cyclinB1、cdk2 /cyclinE和cdk4/cyclinD1,并随病变进展其表达程度增高。B组造成外膜炎性细胞浸润也比对照组明显 ,纤维化程度也高 ,也明显表达cdk’s/cyclins;内、外膜增生程度大致相当 ,动脉硬化的发生较急性排斥晚 ,进展缓慢。滋养血管在A、B两组早期表达cdk’s和cyclins,而且炎性浸润愈重 ,其表达愈?
Objective By means of the rat abdominal aortic allograft model,the purpose of this study is further to consider the vascular smooth muscle cells(VSMC)proliferating regulation in the course of allograft ateriosclerosis(AA)formation.Methods Inbred male Wistar rats served as donors and SD rats as recipients weighed 120～350g.There are three groups.A group:(acute rejection group),90 rats were killed postoperatively in 2,4 and 8 weeks.B group:(chronic rejection group),72 rats were killed in 4,8 and 12 weeks after operation.C group:(native control group)The rat abdominal aortic allograft model was used.Paraffin sections were stained with hematoxylin and eosin(HE),Masson and immunohistochemistry.HE staining was used to observe rejective degree in vessels;masson trichrome coloration was used to judge arteriosclerostic extent;and immunohistochemistry staining,to look into the number of α actin positive expression in VSMC,and those cells expressing degree in cdk1/cyclinB1,cdk2/cyclin E,cdk4/cyclinD1.Results The most obvious lesion in A group was the intense interstitial adventitial infiltration by lymphocytes and mononuclear cells in a large ring around the vessels.At beginning,the inflammatory infiltration were mainly located in the outline of middle and adventitial membrane.Following time and course progressing,the number of α actin positive cells in adventitia were increasing significantly.Those α actin positive cells and vasa vasorum expressed PCNA,cdk's/cyclins remarkably,and all of endogenous regulatory proteins expressine degree were more higher followed by AA progression.The arteriosclerosis in chronic rejective group progressed slowly than A group,and intimal proliferation were equal to advertitia;VSMC in intimal and advertitia were all expressed cdk's/cyclins.In A and B groups,vasa vasorum early expressed α actin,PCNA,cdk's and cyclins.Following inflammatory infiltration accelerately,they expressed more early and obviously.If vasa vasorum proliferated more fast and earlier,their caliber would be steno
出处
《中国公共卫生》
CAS
CSCD
北大核心
2001年第7期601-603,共3页
Chinese Journal of Public Health
基金
山东省科学技术厅立项项目 (编号 :951 2 2 81 0 8)
