摘要
目的 观察重组人白介素１０（ｒｈＩＬ－１０）对血管平滑肌细胞（ＶＳＭＣ）增殖的影响。方法 离体培养大鼠胸主动脉 ＶＳＭＣ并分别接受不同浓度ｒｈＩＬ－１０、肿瘤坏死因子（ＴＮＦ－α）、血小板源性生长因子（ＰＤＧＦ－ＢＢ）的刺激，采用ＭＴＳ／ＰＥＳ 法确定ＶＳＭＣ的增殖状态并与对照组比较。结果 与对照组相比，ＴＮＦ－α和ＰＤＧＦ－ＢＢ均对ＶＳＭＣ增殖具有明显的刺 激作用（Ｐ＜０．０５）。单独应用ｒｈＩＬ－１０对血管平滑肌细胞生长没有影响。在ＴＮＦ－α和ＰＤＧＦ－ＢＢ分别刺激下，ｒｈＩＬ－１０均可 抑制ＶＳＭＣ的生长（Ｐ<０．０５）。结论 ｒｈＩＬ－１０可抑制细胞因子和生长因子诱导的ＶＳＭＣ增殖。
Objective To observe the effects of recombinant human interleukin-10 (rhIL-10) on the proliferation of rat vascular smooth muscle cells (VSMCs) cultured in vitro. Method Aortic VSMCs cultured in vitro were treated with varied doses of rhlI-10 alone or in combination with tumor necrosis factor-a (TGF-a) or platelet-derived growth factor-BB(PDGF-BB) respectively. The VSMC proliferation was quantified by colormetric assay. Results Compared with control, both TNF-a and PDGF-BB stimulated conspicuous proliferation of VSMCS, bit the effect of which was not observed by rhIL-10 treatment alone. In the presence of rhlL-10, the proliferation effects of both TGF-a and PDGF-BB on VSMCs were significantly inhibited (P<0.05). Conclusion rhIL-10 can inhibit VSMC proliferation induced by TNF-a and PDGF-BB, thus may provide a new therapeutic approach for regulating vascular wall remodeling after vascular injury.
出处
《第一军医大学学报》
CSCD
北大核心
2001年第11期828-830,共3页
Journal of First Military Medical University
基金
广州市科委科技攻关项目(JB00000448165)
