摘要
目的 探讨血管内皮生长因子 (VEGF)基因心肌细胞转移促进异种生物瓣膜宿主内皮化的可行性。方法 将戊二醛及L 谷氨酸处理过的牛心包置入猪的右心房 ,采用pcD2 /hVEGF12 1基因缝线向右室心肌细胞转移VEGF基因 (1mg/只 )。测定外周及右房血中VEGF蛋白含量 ,应用逆转录聚合酶链反应 (RT PCR)测定心肌中VEGF基因的表达 ,并取置入的牛心包进行组织学及超微形态学分析。结果 转pcD2 /hVEGF12 1基因 10d后 ,基因组右房血中VEGF蛋白含量明显高于空载质粒组 (P<0 .0 1) ,术后 16d ,基因组心肌VEGFmRNA表达产物明显高于空载质粒组及左室对照组。此间 ,VEGF基因组牛心包宿主内皮覆盖率亦显著高于质粒组 (P <0 .0 5 ) ,术后 30d ,VEGF基因组牛心包已完全内皮化。结论 采用基因缝线心肌细胞转移VEGF基因 ,可防止生物瓣膜钙化 。
Objective To investigate the feasibility of host endothelialization of transplanted hetero heart valve by transfer of VEGF gene. Methods Bovine pericardium treated with glutaraldehyde and L glutamine was positioned into ping's right atrium, then gene suture carrying plasmids with pcD 2/hVEGF 121 gene or only with pcD 2 was sewn into the anterior wall of right ventricle. The contents of VEGF protein in blood from right atrium and peripheral vein were deteremined by ELISA 10 days after the tranplantation. The expression of VFGF mRNA in the myocardium near the gene suture was detected by RT PCR 16 days after the tranplantatipn. Microscopy and unltrastructural analysis of the tranplanted valve were carried out. Results The content of VEGF protein in blood from the right atrium in the pcD 2/hVEGF 121 group was significantly higher than that in the pcD 2 group 10 days after the VEGF gene transfer ( P <0.01). The expression of VEGF mRNA in the myocardium of right ventricle in pcD 2/VEGF121 group was much higher than that in the left ventricle of the same group and that in the right ventricle in pcD 2 group. Morphological observation showed that the coverage rate of host endothelium in pcD 2/hVEGF 121 group was higher than in pcD 2 group 16 days after the gene trasfer ( P <0.05). 30 days after the gene transfer. Complete host endothelialization was observed 30 days after the operation. Conclusion VEGF gene transfer with surgical suture promotes the host endothelialization of bioprothesis, thus inhibiting calcification of biological valves and improving biocompatibility and long term durability of bioprothesis.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2001年第17期1074-1077,共4页
National Medical Journal of China
基金
江苏省自然科学基金资助项目 (98K83 2 0 0 0 8)
