摘要
目的研究 CTL A- 4Ig在治疗 C5 7BL/ 6小鼠自身免疫性肝炎上的作用。方法通过用 C5 7BL/ 6近交系小鼠的肝特异性抗原与弗氏完全佐剂混合物免疫攻击小鼠 ,随后用 CTL A- 4Ig治疗 ,观察小鼠的临床经过、血生化、肝脏组织学改变。结果随着免疫次数的增多 ,治疗组临床经过、血生化、肝脏组织学改变逐步与正常对照组相似 ,而病理模型组与前两组有明显差异 :血生化可见天冬氨酸氨基转移酶、球蛋白显著升高 ;肝组织学检测可见炎细胞的浸润 ,肝细胞的肿胀、灶性坏死甚至广泛坏死 ;肝组织免疫荧光检测提示有大量免疫球蛋白沉着。结论 CTL A- 4Ig能有效地治疗 C5 7BL /
ObjectiveTo investigate the role of cytotoxic T lymphocyte associated molecule 4Ig(CTLA 4Ig) in treatment autoimmune hepatitis in C57BL/6 mice Methods C57BL/6 mice were intraperitoneally immunized with C57BL/6 mice liver specific protein in complete Freund's adjuvant, CTLA 4Ig were subsequently given Clinical conditions, serum biochemistry alteration of liver histology of C57BL/6 mice were observed Results With sequential immunization, the clinical, serum biochemistry, liver histological changes in treated group were similar to those of the control group; In the pathological model group, aspartate aminotransferase and globulin significantly increased Inflammatory cell infiltration, hepatic cell swelling, focal necrosis and severe hepatocyte necrosis were found in liver; and liver immunofluorescence detection cued that there were many immunoglobulin deposited there, it is significantly different to the other two groups Conclusion CTLA 4Ig can treat C57BL/6 mice autoimmune hepatitis
出处
《免疫学杂志》
CAS
CSCD
北大核心
2001年第6期453-456,共4页
Immunological Journal
基金
国家自然科学基金重点项目 (39993430 - 2 )
国家自然科学基金面上项目 (39970 75 6 )
国家科技部重点项目 (96 -92 0 - 2 0 - 10 )
重庆市科委院士基金 (96 90 10 5 18)资助项目
