摘要
利用Sevag法脱游离蛋白、乙醇沉淀、二次透析等手段得到了初步纯化的茶叶粗多糖,该粗多糖经SephadexG100凝胶柱层析纯化,0.1mol/LNaCl洗脱,获得一较大组分,命名为TGP,通过凝胶渗透法测定其平均分子量为6×104道尔顿。
In this paper, coarse tea polysaccharides were preliminarily purified by means of Sevag deprotein, ethanol precipitation, two times dialysis etc. A large component, nominated TGP, was fractionated from the polysaccharides by Sephadex G100 gel column chromatography, eluted by 0.1mol/L NaCl. The average molecular weight was determined to be 6.0× 104 Dol by gel permeation chromatography.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2001年第11期46-47,共2页
Food Science
基金
教育部<高等学校骨干教师资助计划>项目及<江西省主要学科跨世纪学术和技术带头人培养计划项目>资助。
关键词
茶叶粗多糖
凝胶柱层析
TGP
分子量
提取
纯化
Coarse tea polysaccharides Gel column chromatography TGP Molecular weight
