摘要
目的 研究沙棘油乳剂中的α 亚麻酸 (α LNA)在大鼠体内的吸收、分布、生物利用度及蛋白结合率等药动学特征。方法 沙棘油乳剂中的α LNA在生物样品中的浓度用气相色谱法测定。结果 大鼠静注及恒速静脉输注沙棘油乳剂后 ,其药 时过程均符合开放二室模型特征 ;大鼠灌胃给予沙棘油及沙棘油乳剂后 ,药 时曲线均符合开放性单隔室一级吸收模型 ;沙棘油和沙棘油乳剂的绝对生物利用度分别为 34 .9% ,6 4.8%。沙棘油乳剂与沙棘油油剂相比 ,α LNA的相对生物利用度为185 .7% ,静脉注射 3个不同剂量的沙棘油乳剂后 (α LNA分别为 2 6 .4,5 2 .8,10 5 .8mg·kg-1) ,其药时过程符合二室开放模型特征 ,且 3个剂量间的消除半衰期及AUC与计量之比无显著性差异 ,具有线性动力学特征。血浆蛋白结合率为 95 .32 %。体内分布实验结果表明α LNA在体内分布较广泛 ,主要集中在肝、脾 ,在大鼠小鼠体内的分布存在差异。结论 该研究为沙棘油及α
OBJECTIVE: To study the absorption, distribution, bioavailability and protein binding of the α-linolenic acids (α-LNA) in Hippophae Oil emulsion in rats. METHODS: A GC assay was used to determine the α-LNA concentration in biological samples. RESULTS: The concentration-time profiles of α-LNA in rats after iv and iv infusion of Hippophae oil emulsion both were shown to fit a two-compartment open model. The concentration-time profiles of α-LNA fitted one-compartment open model with first-order absorption after oral administration. The absolute bioavailability of Hippophae oil and Hippophae oil emulsion were 34.9% and 64.8%, respectively. And the relative bioavailability of Hippophae oil emulsion was 185.7%. The concentration-time profiles of α-LNA in rats after iv infusion of 26.4,52.8,105 mg·kg-1 were all shown to fit the two-compartment open model. There were no significant difference of the disposition among these three dosages,and it was found linear kinetics. The α-LNA distributed widely in rats and mice, especially in liver and spleen, with significant differences. The plasma protein binding was about 95%. CONCLUSION: This study provided a scientific basis for the further study and the application of α-LNA and hippophae oil.
出处
《中国药学杂志》
EI
CAS
CSCD
北大核心
2001年第9期612-615,共4页
Chinese Pharmaceutical Journal
