摘要
目的 :探讨糖尿病大鼠肺诱生型一氧化氮合酶 (iNOS)及血管活性肠肽 (VIP)的病理变化。方法 :采用免疫组织化学方法 ,观察正常和四氧嘧啶诱导的糖尿病 4周大鼠肺内iNOS及VIP的变化 ,并进行图像分析。结果 :糖尿病 4周大鼠肺支气管上皮细胞、肺泡管上皮细胞、肺泡囊上皮细胞和动脉内皮细胞iNOS呈阳性或弱阳性 ,静脉内皮细胞、毛细血管内皮细胞iNOS呈阴性。图像分析表明 ,糖尿病鼠肺iNOS的着色面积、积分光密度和相对含量均明显低于正常大鼠肺。糖尿病 4周鼠肺支气管纤毛上皮细胞VIP呈阳性 ,支气管平滑肌肌层、支气管粘膜下腺体周围、肺间隔和肺动脉壁外膜VIP呈弱阳性或阴性 ,图像分析显示糖尿病鼠肺VIP着色面积、积分光密度和相对含量均明显低于正常大鼠肺。结论 :糖尿病肺病变不仅受自主神经影响 ,而且非肾上腺素能非胆碱能神经改变亦与之有关 ,其神经递质NO和VIP可能在糖尿病肺病变中具有重要意义。
AIM: The pathological changes in inducible nitric oxide synthase (iNOS) and vasoactive intestinal peptide (VIP) in diabetic rat lung were investigated.METHODS: Using immunohistochemical method and imaging analysis, the changes in iNOS and VIP were observed in normal and diabetic rat lung. RESULTS: In diabetic rats, the iNOS positive or weak positive staining was localized in epithelial cells of bronchi, epithelial cells of alveolar ducts, alveolar sacs, and arteries, but negative in endothelial cells of vein and capillary. Image analysis showed the area, the integral absorbance(IA) and relative contents of iNOS were significant lower than that of control (P<0.01, P<0.01 and P<0.01). In diabetic rats, the ciliated cells of bronchi epithelium showed VIP positive reaction, while the bronchial smooth muscle, surounding tracheobronchial submucosal glands, the tunica adventitia of pulmonary arteries, and alveolar septum were stained weak positive or negative for VIP. Image analysis showed the area, the IA and relative contents of VIP were significantly lower than those of control (P<0.01, P<0.01 and P<0.01).CONCLUSION: The pathological changes in diabetic rat lungs are not only involving autonomic neuropathy but also NANC nerves. Nitric oxide and VIP, which are NANC neurotransmitter, may play an important role in pathogenesis of pathological change in diabetic lung.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2001年第10期1008-1011,T002,共5页
Chinese Journal of Pathophysiology
