摘要
利用大鼠促性腺激素释放激素放射免疫学测定试剂检测了根田鼠下丘脑促性腺激素释放激素水平 ,测定线性范围为 2 5pg至 1 60pg/管 ,批内和批间差为 1 7% (n =1 0 )和 7 8% (n =4) ,样品平均标准回收率为 1 0 8 2± 8 4%。根田鼠下丘脑促性腺激素释放激素测定为 0 2 8± 0 0 4ng/mg湿重组织。从根田鼠下丘脑提取物稀释曲线与大鼠 (人工合成 )促性腺激素释放激素有较好的平行关系判断 ,可以推知根田鼠下丘脑促性腺激素释放激素结构活性类似于大鼠 (人工合成 )
The contents of the hypothalamic gonadotrophin releasing hormone (GnRH ) of the root vole( Microtus oeconomus ) were successfully tested by using a rat GnRH and its antiserum with a sensitivity range of 2 5 pg to 160 pg /tube Intra and inter assay coefficient of variation were 1 7%(n=10) and 7 8% (n=4), respectively The extract of hypothalamus of the vole showed a good parallel dose response curve with synthetic rat GnRH The rate of average standard recovery was 108 2±8 4% The mean levels of hypothalamic GnRH of the voles were 0 28±0 04 ng/mg wet weight tissue The results suggest that the hypothalamus of the vole contains a GnRH similar to rat GnRH
出处
《兽类学报》
CAS
CSCD
北大核心
2001年第4期287-291,共5页
Acta Theriologica Sinica
关键词
促性腺激素释放激素
放射免疫学测定
根田鼠
下丘脑
Gonadotrophin releasing hormone
Radioimmunoassay
Root vole ( Microtus oeconomus )
Hypothalamus
