摘要
对高产菌株黑曲霉I11—N14的产酶、发酵条件以及金属离子和表面活性剂对产酶的影响研究表明,最适发酵条件为:发酵96h、摇床转速240r/min、添加单宁酸2.00%、pH5.5和温度30℃。在培养基中加入适量Mn2+,Sn2+可促进单宁酶的产生。表面活性剂EDTA抑制产酶,而二甲苯和SDS对产酶有一定的刺激作用。
The synthetic curve of producing tannase in Aspergillus niger I 11 —N 14 and the optimum ferment conditions of I 11 —N 14 strain as well as the effects of metal ions and surfactant solutions against tannase production were studied. The results revealed that the optimum ferment conditions were as follows: ferment time 96 hours, rotatory speed 240 r/min, tannin acid 2.00%, pH value 5.5 and temperature 30℃. Mn 2+ and Sn 2+ contained in cultural basement could help to increase tannase production and surfactant EDTA could hold tannase production but dimethybenzene and SDS had certain stimulation of producing tannase.
出处
《西南农业大学学报（自然科学版）》
CSCD
1999年第5期433-436,共4页
Journal of Southwest Agricultural University
