摘要
目的 探讨补体 C5与中性粒细胞蛋白酶之间的相互作用，研究血液透析时中性粒细胞被激活的机制。 方法 通过免疫凝胶电泳 (SDS－ PAGE)和免疫印迹法检测 C5或 C5片段，用酶联免疫吸附 (ELISA)检测白细胞释放的组织蛋白酶 G、弹性酶和乳铁传递蛋白 (Lactoferrin)；用色谱分析法检测白细胞释放的髓过氧化酶 (MPO)。 结果 C5暴露于组织蛋白酶 G和弹性酶时，产生 C5片段，而 C5片段能使活化的白细胞释放 MPO和乳铁传递蛋白。随着弹性酶浓度增加， C5片段导致中性粒细胞释放 MPO和乳铁传递蛋白增加，而组织蛋白酶 G对两者的释放产生双向反应。结论 本研究结果表明 C5片断对刺激中性粒细胞脱颗粒有重要作用。
Objective To study the effect of neutrophil granular proteases on the complement C5 activation to clarify the mechanisms of neutrophil stimulation during hemodialysis. Methods C5 or C5 fragments were measured by SDS－ PAGE and immuno blotting.cathepsin G,elastase,and lactoferrin were measured using a two site enzyme－ linked immunosorbent assay(ELISA).MPO was measured using a chromogenic assay. Results C5 exposed to cathepsin G and elastase generated C5 fragments.C5 fragments stimulated primed neutrophil to release myeloperoxidase(MPO) and lactoferrin.C5 fragment induced MPO and lactoferrin release was produced in an incremental manner with elastase,however a biphasic response with cathepsin G. Conclusion Our results suggest that C5 fragment is important to stimulate neutrophils degranulation.
出处
《罕少疾病杂志》
2001年第2期12-14,共3页
Journal of Rare and Uncommon Diseases
