摘要
目的 探讨大鼠实验性急性胰腺炎 (AP)模型中胰蛋白酶原激活肽 (TAP)的产生及意义。方法 将 90只SD大鼠随机分为 5组 :EP组 ( 3%牛磺胆酸钠逆行胆胰管注射组 ) ;NP组 ( 5%牛磺胆酸钠逆行胆胰管注射组 ) ;TP组( 3%牛磺胆酸钠逆行胆胰管注射后半小时经股静脉注入乌司他丁组 ) ;CP组 ( 0 .9%生理盐水逆行胆胰管注射组 ) ;OP组 (假手术组 )。分别于制模后 3、6及 2 4小时后处死动物 ,取血测定血淀粉酶和TAP水平 ,同时取胰腺标本观察其组织病理学改变并评分。结果 NP组胰腺病变明显重于EP组。制模后 3小时和 6小时血浆TAP水平NP组分别为( 4 .798±0 .1 6 9)nmol/L和 ( 3.999±0 .2 99)nmol/L ,明显高于EP组的 ( 2 .41 6 ±0 .1 48)nmol/L和 ( 3.356 ±0 .2 1 1 )nmol/L ;在制模 6小时后TP组血浆TAP水平为 ( 1 .6 1 1 ±0 .1 1 3)nmol/L ,比EP组的 ( 3.356 ±0 .2 1 1 )nmol/L明显降低。EP组与NP组血浆TAP水平差异的出现早于两组间胰腺组织病理学改变差异的出现。结论 血浆TAP水平与大鼠实验性AP的严重程度有关。血浆TAP水平可以作为早期预测实验性AP严重程度的指标。
Objective To investigate the production and possible significance of plasma trypsinogen activation peptides (TAP) in rat experimental acute pancreatitis. Methods Ninety SD rats were randomly allocated to five groups: group EP with retrograde ductal infusion of 3%sodium taurocholate; group NP with retrograde ductal infusion of 5%sodium taurocholate; group TP with retrograde ductal infusion of 3%sodium taurocholate and ulinastatin(UTI) intravenous infusion half an hour later; group CP with 0.9% NS retrograde ductal infusion; group OP with sham operation. Animals in each group were killed 3h,6h and 24h after infusion. Plasma TAP was determined by EIA.The histological severity of the pancreas were assessed by Schmidt method. Results The pancreatic pathological changes in group NP was significantly severe than in group EP. At 3h and 6h after infusion, plasma TAP concentration of group NP (4.798±0.169)nmol/L and (3.999±0.299)nmol/L were significant higher than that of group EP(2.416±0.148)nmol/L and (3.356± 0.211) nmol/L. At 6h after infusion plasma TAP concentration of group TP 〔(1.611±0.113)nmol/L〕 was significant lower than that of group EP(3.356±0.211)nmol/L. The difference of plasma TAP concentration between group EP and group NP appeared prior to the difference of the histopathological changes of pancreas between two groups. Conclusion Plasma TAP concentration is connected with the severity of sodium taurocholate induced rat pancreatitis. Plasma TAP concentration may be used as a marker for early assessment of the severity of this experimental acute pancreatitis.
出处
《中国普外基础与临床杂志》
CAS
2001年第5期301-303,306,共4页
Chinese Journal of Bases and Clinics In General Surgery
