摘要
本试验利用PCR -RFLPs技术 ,用MspⅠ ,ApaⅠ两种限制酶 ,检测了猪生长激素基因 +2 0 6～ +711位共5 0 6bp片段中的 [+2 95～ +30 0、+5 6 3～ +5 6 6 ]2处突变位点 ,分析了各多态位点在姜曲海猪中的分布及其与生产性能的关系。发现 :姜曲海猪中 ,MspⅠ酶切突变位点中的G1G2基因型和G2G2基因型个体的生产性能没有差异 ,不同日龄体重在两组间变化没有规律。ApaⅠ酶切突变位点G4G4基因型个体的 2 0日龄体重、45日龄体重高于G3G3/G3G4基因型个体 ,但差异不显著 ,G4G4基因型个体的 70日龄体重极显著高于G3G3/G3G4基因型个体 (P <0 .0 1)。G4G4基因型个体的 0～ 70日龄日增重也极显著高于G3G3/G3G4基因型个体 (P <0 .0 1)。这些结果显示GH基因有可能作为数量性状基因座的侯选基因。
A 506bp segment of the porcine growth hormone gene encompassing base +206 of the first intro through base +711 of the third exon was amplified by PCR[Base numbering followed the designation of Vice & Wells (1987)] . Two polymorphic restriction sites for ApaⅠ,MspⅠ were identified at +295～+300,+563～+566 respectively. DNA was from Jianquhai breeds?The effect of genotype on bodyweight was analysed .The result showed that there was no difference of bodyweight between G1G2 and G2G2 genotype pigs in MspⅠ polymorphic restriction sites. But in Apa Ⅰ polymorphic restriction sites, the 70-days-bodyweight and average day gain from birth to 70-days of G4G4 genotype pigs was extremely significant( P <0.01) higher than G3G4 /G3G3 genotype pigs.Result suggested that GH gene may be a candidate gene for quantitative trait locus in pigs.
出处
《遗传》
CAS
CSCD
北大核心
2001年第5期427-430,共4页
Hereditas（Beijing)
