摘要
家蚕抗菌肽CM_4可抑制串珠镰孢菌孢子的生长发育。扫描电镜超微结构观察显示孢子空泡化,内部结构萎缩成团,对照组的孢子结构则完好无损,发育正常。家蚕抗菌肽与真菌作用,用激光共聚焦扫描图象分析,发现荧光素FITC标记抗菌肽先是聚集包围细胞,呈中间浓度大,两头浓度小状态分布,进而两头断裂,或形成空泡,最后连空泡的膜也破裂,细胞死亡,形成一堆碎片。
The antibacterial peptide CM) inhibits growth of conidia of Fusaricum moniliforme. The observation by using the electron microscope showed that some cells were empty and interior structure twisted into helix. Conidia cells in control group were in good condition, nornally grew up. The fungal conidia treated with the antibacterial
peptide CM4, then confocal laser scanning microscope were performed. As a result, it was showed that FITC-labeled peptide assembled in cells, centre of which had more pep-tides than two ends. Then conidia splil, became empty. Finally the cells plasma membrane disintegrated and died, lead to small fragments.
出处
《实验生物学报》
SCIE
CAS
CSCD
北大核心
2001年第3期177-181,共5页
Acta Biologiae Experimentalis Sinica
基金
国家自然科学基金(批准号:39770117)
关键词
家蚕
昆虫抗菌肽
CM4
串珠镰孢菌子
抗真菌作用
antibacterial peptide CM4. Fusaricum moniliforme. infrastructure, confocal laser scanning micro-scope(CLSM)
