摘要
为了探讨成年猴雪旺氏细胞的在体增殖和体外迁移的能力,我们对用神经结扎术结扎的A组6只3-13岁雄性恒河猴的腓肠神经进行植块培养,部分细胞培养在聚酯纤维上,2-4周后作抗S-100抗体免疫组化染色和电镜观察;B组2只未做结扎的新生猴腓肠神经培养作为对照。结果显示:A组雪旺氏细胞平均在培养的第5天从神经段中迁出,年幼者早于成年猴;细胞在纤维上以螺旋状向前迁移;雪旺氏细胞抗S-100蛋白抗体染色阳性;电镜显示,雪旺氏细胞包卷纤维,但是,未见髓鞘形成。B组神经段培养2周仍无雪旺氏细胞迁出。研究表明,结扎神经使其发生瓦勒氏变性,经植块培养、纯化,能够获得可用于移植的成年猴的雪旺氏细胞。
For the purpose of study the adult monkey Schwarm Cell (SC) proliferation in vivo and migration in vitro, the sural nerve of six monkeys in 3 to 13 years old were tied up for 7 days, then, the distal segment of sural nerve were cultured as group A. Some SCs of group A were cultured on the polyester fiber to observe the migration of the cells. SCs were observed by SEM and TEM after 2-4 weeks in vitro. Meanwhile, the normal sural nerve of 2 newborn monkeys were cultured as group B. Results showed as follows: The time of SC migration from nerve segment of group A was earlier in young monkey than in older one. SEM and TEM showed that the SCs migrated in a spiral fashion on the polyester fiber and wrapped if, there was not any SC migration from segment of group B after 2 weeks in vitro. Results suggested that following the nerve ligature to induce the nerve regeneration in vivo, the adult monkey SC may be harvested with the procedure of nerve tissue culture.
出处
《细胞生物学杂志》
CSCD
北大核心
2001年第3期182-184,F003,共4页
Chinese Journal of Cell Biology
基金
复旦大学医学院神经生物学国家重点实验室
中科院上海生理所开放实验室资助
课题号010113
