摘要
目的 观察蜂毒肽对新生大鼠培养心肌细胞Na+,K+ ATP酶α1和 β1亚基mRNA的表达。方法 采用逆转录聚合酶链反应 (RT PCR)技术。药物与培养的心肌细胞温育 1h后 ,观察蜂毒肽对Na+,K+ ATP酶基因表达的影响。用GAPDHmRNA作为内参照。 结果 蜂毒肽对α1亚基mRNA表达有显著促进作用。经GAPDH校正 ,光密度值对照组为 0 .47± 0 .14(n =5 ) ,蜂毒肽 0 .0 1mmol/L组为 0 .6 6± 0 .10 (n =6 ,P <0 .0 5 ) ,哇巴因 1mmol/L组为 0 .6 7± 0 .0 9(n =6 ,P <0 .0 5 )。蜂毒肽 0 .0 1mmol/L组和哇巴因 1mmol/L组对Na+,K+ ATP酶β1亚基mRNA表达无显著影响。结论 蜂毒肽 0 .0 1mmol/L对Na+,K+ ATP酶α1亚基mRNA表达有促进作用 ,但对
Purpose: To observe the effect of melittin (Mel) on the expression of Na+, K+-ATPase subunit mRNA in cultured neonatal rat myocardial cells. Methods: Na+, K+-ATPase α 1 and β1 isoforms mRNA level were observed with reverse transcriptase polymerase chain reaction (RT-PCR). The drugs were presented in medium for 1 h after process of cell culture. GAPDH mRNA level was used as an inner standard. Results: Mel of 0.01 mmol/L and Ouabain of 1 mmol/L increased the level of mRNA α1from 0.47 ± O.14 (n = 5) to O.66 ± O.1O (n = 6, P1 did not change significantly after administration of Mel or Ouabain in the same dose mentioned above. Conclusions: Mel increased the mRNA level of Na+, K+-ATPase α1 isoform, but did not change that of β1 isoform.
出处
《复旦学报（医学版）》
EI
CAS
CSCD
北大核心
2001年第4期340-342,346,共4页
Fudan University Journal of Medical Sciences
基金
国家自然科学基金 (39770 885 )资助项目
