摘要
我们用菌落免疫荧光染色技术对4株(3D_1、3A_(11)、3F_5、2E_5)抗精氨酸支原体(MycoPlasma arginini)McAb进行了鉴定,在热水固定的菌落上3D_1、3A_(11)不仅与精氨酸支原体出现了阳性反应,而且与口腔支原体(M.orale)、猪鼻支原体(M.hyorhinis)、莱氏无胆甾原体(AcholePlasma laidlawii)、羊无乳支原体(M.agalactia)PG_2和牛丝状支原体丝状亚种(M.mycoides subsp. mycoides)PG_1等均有交叉反应;在未固定的菌落上用表面免疫荧光检测时, McAb却表现了与PcAb不同的特性。同时我们还做了阻断试验,进一步证明了我们制备的McAb的支原体特异性。
Four monoclonal antibodies (3D_1, 3A_(11), 3F_5 and 2E_5) against MyaoPlasma arginini were identified with colony IFA technique. At the colonies fixed by the hot water, 3D_1 and 3A_(11) not only revealed positive reaction with M. arginini, but also crossly reacted with M. orale, M. hyorhinis, A. laidlawii, M. agalactia (PG2), and M. mycoides subsp, myeoides (PG1). But at the unfixed colonies, the monoclonal antibodies had different characteristics from polyclonal antibodies. The results obtained by blocking test demonstrated the specificity of our prepared McAb against mycoplasma.
出处
《军事医学科学院院刊》
CSCD
北大核心
1991年第3期172-175,共4页
Bulletin of the Academy of Military Medical Sciences
关键词
支原体属
单克隆抗体
菌落染色
mycoplasma
monoclonal antibodies
colony IFA technique
