摘要
用ＨＰＬＣ／ＥＣＤ法测定血小板５－ＨＴ，运用 ＷａｔｅｒｓμＢｏｎｄａｐａｋ Ｃ柱，３，４二羟基苄胺（ＤＨ－ＢＡ）作内标，以 ０.０７ ｍｏｌ／Ｌ乙酸钠缓冲液＋乙腈为流动相（２０＋１），每１Ｌ缓冲液含 ０．０５ ｍｏｌ／Ｌ柠檬酸、２．５ ｍｍｏｌ／Ｌ庚烷磺酸钠、０．１ｍｍｏｌ／Ｌ Ｎａ_２ＥＤＴＡ，流速０．８ ｍＬ／ｍｉｎ。ＤＨＢＡ和 ５－ＨＴ的保留时间分别为５．４和１１．５ ｍｉｎ，线性范围为０．０２５～１ｍｇ／Ｌ（ｒ＝ ０．９９９７）。５－ＨＴ日内 ＲＳＤ低于１．８７％，日间ＲＳＤ低于８．５４％，方法回收率为１００．４％±２．３％。提示本方法快速简便，灵敏准确，适用于临床基础研究。
A sensitive and rapid method was reported for the determination of 5-HT in patients platelets by using high-performance liquid chromatography with an electrochemical detector (HPLC-ECD) and Wa- ters μBondapak column(10μ). 3, 4-Dihydroxybenzylamine (DHBA )was used as an internal standard .The mobile phase of 0 .07mol/L sodium acetate buffer (pH4.0) + acetonitrile (20 + 1 ) with of 2 .5mmol/L sodium 1-heptanesulfonate, 0. 1mmol/L EDTA-Na_2, 0.05mol/L citric acid was pumped through the col- umn with a flow rate of 0. 8mL/min, .The length of retention time for DHBA and 5-HT was 5 .4 min and 11. 5 min. The standard curve was linear within the concentration range of 0. 025 to 1mg/L for 5-HT(r = 0.9997) .The within day RSD (% ) was less than 1. 87% for 5-HT, inter-day RSD (% ) was less than 8. 54% for 5-HT .The average recovery (% ) for 5-HT was 100 .4 ±2. 3% .This method has been applied to clinical research.
出处
《广东微量元素科学》
CAS
2001年第5期50-52,共3页
Trace Elements Science
